Part:BBa_K1641037:Design

Inductive invertase generating vector: pInv-gen-Flp-EGFP-ssra

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1205
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1144
  Illegal XhoI site found at 1740
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 979
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 961
  

Design Notes

All invertase generators are constructed as following structure:

AraC+Pbad::RBS::invertase-EGFP(-ssra)::terminator

Source

This brick is constructed through cut-and-link of other bricks without risky sources, usually E. coli originated genes.